Article
Rapid identification of lettuce seed germination mutants by bulked segregant analysis and whole genome sequencing.
The Plant journal : for cell and molecular biology - 1 Nov 2016
Huo Heqiang, Henry Isabelle M, Coppoolse Eric R, Verhoef-Post Miriam, Schut Johan W, de Rooij Han, Vogelaar Aat, Joosen Ronny V L, Woudenberg Leo, Comai Luca, Bradford Kent J
Abstract excerpt
Lettuce (Lactuca sativa) seeds exhibit thermoinhibition, or failure to complete germination when imbibed at warm temperatures. Chemical mutagenesis was employed to develop lettuce lines that exhibit germination thermotolerance. Two independent thermotolerant lettuce seed mutant lines, TG01 and TG10, were generated through ethyl methanesulfonate mutagenesis. Genetic and physiological analyses indicated that these...
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