Article
Analysis of Quasispecies of Avain Leukosis Virus Subgroup J Using Sanger and High-throughput Sequencing.
Virology journal - 27 Jun 2016
Meng Fanfeng, Dong Xuan, Hu Tao, Liu Yingnan, Zhao Yingjie, Lv Yanyan, Chang Shuang, Zhao Peng, Cui Zhizhong
Abstract excerpt
BACKGROUND: Avian leukosis viruses subgroup J (ALV-J) exists as a complex mixture of different, but closely related genomes named quasispecies subjected to continuous change according to the Principles of Darwinian evolution. METHOD: The present study seeks to compare conventional Sanger sequencing with deep sequencing using MiSeq platform to study quasispecies dynamics of ALV-J. RESULTS: The accuracy and...
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