Article
Isolation and characterization of xylitol-assimilating mutants of recombinant Saccharomyces cerevisiae.
Journal of bioscience and bioengineering - 1 Oct 2016
Tani Tatsunori, Taguchi Hisataka, Fujimori Kazuhiro E, Sahara Takehiko, Ohgiya Satoru, Kamagata Yoichi, Akamatsu Takashi
Abstract excerpt
To clarify the mechanisms of xylitol utilization, three xylitol-assimilating mutants were isolated from recombinant Saccharomyces cerevisiae strains showing highly efficient xylose-utilization. The nucleotide sequences of the mutant genomes were analyzed and compared with those of the wild-type strains and the mutation sites were identified. gal80 mutations were common to all the mutants, and recessive to the...
Topics
- Aldehyde Reductase
- Batch Cell Culture Techniques
- Fermentation
- Gene Deletion
- Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating)
- Hydrogen-Ion Concentration
- Monosaccharide Transport Proteins
- Mutation
- Oxygen
- Phenotype
- Repressor Proteins
- Saccharomyces cerevisiae
- Saccharomyces cerevisiae Proteins