Article
A TaqMan real-time PCR assay for detection and quantification of Sporisorium scitamineum in sugarcane.
TheScientificWorldJournal - 1 Jan 2013
Su Yachun, Wang Shanshan, Guo Jinlong, Xue Bantong, Xu Liping, Que Youxiong
Abstract excerpt
Sporisorium scitamineum is a fungal smut pathogen epidemic in sugarcane producing areas. Early detection and proper identification of the smut are an essential requirement in its management practice. In this study, we developed a TaqMan real-time PCR assay using specific primers (bEQ-F/bEQ-R) and a TaqMan probe (bEQ-P) which were designed based on the bE (b East mating type) gene (Genbank Accession no. U61290.1)....
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