Article
Genome-wide generation and use of informative intron-spanning and intron-length polymorphism markers for high-throughput genetic analysis in rice.
Scientific reports - 1 Apr 2016
Badoni Saurabh, Das Sweta, Sayal Yogesh K, Gopalakrishnan S, Singh Ashok K, Rao Atmakuri R, Agarwal Pinky, Parida Swarup K, Tyagi Akhilesh K
Abstract excerpt
We developed genome-wide 84634 ISM (intron-spanning marker) and 16510 InDel-fragment length polymorphism-based ILP (intron-length polymorphism) markers from genes physically mapped on 12 rice chromosomes. These genic markers revealed much higher amplification-efficiency (80%) and polymorphic-potential (66%) among rice accessions even by a cost-effective agarose gel-based assay. A wider level of functional...
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