Article
Screening for major driver oncogene alterations in adenosquamous lung carcinoma using PCR coupled with next-generation and Sanger sequencing methods.
Scientific reports - 29 Feb 2016
Shi Xiaohua, Wu Huanwen, Lu Junliang, Duan Huanli, Liu Xuguang, Liang Zhiyong
Abstract excerpt
We investigated the frequency of major driver oncogenes in lung adenosquamous cell carcinoma (ASC) cases. Frequency of EGFR, K-Ras, B-Raf, PIK3CA, DDR2, ALK, and PDGFRA gene mutations was examined in 56 patients using next-generation sequencing, polymerase chain reaction, and Sanger sequencing. Macrodissection or microdissection was performed in 37 cases to separate the adenomatous and squamous components of ASC....
Topics
- Adenocarcinoma
- Adult
- Aged
- Aged, 80 and over
- Carcinoma, Squamous Cell
- Cluster Analysis
- ErbB Receptors
- Female
- Gene Expression Profiling
- Genes, ras
- High-Throughput Nucleotide Sequencing
- Humans
- Kaplan-Meier Estimate