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Development of Real-Time PCR Methods for the Detection of Bacterial Meningitis Pathogens without DNA Extraction

1 Feb 2016

Abstract excerpt

Neisseria meningitidis (Nm), Haemophilus influenzae (Hi), and Streptococcus pneumoniae (Sp) are the lead causes of bacterial meningitis. Detection of these pathogens from clinical specimens using traditional real-time PCR (rt-PCR) requires DNA extraction to remove the PCR inhibitors prior to testing, which is time consuming and labor intensive. In this study, five species-specific (Nm-sodC and -ctrA, Hi-hpd#1 and...

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PMID: 26829233
DOI: 10.1371/journal.pone.0147765

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