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New Primers Targeting Full-Length Ciliate 18S rRNA Genes and Evaluation of Dietary Effect on Rumen Ciliate Diversity in Dairy Cows.

Current microbiology - 1 Dec 2015

Zhang Jun, Zhao Shengguo, Zhang Yangdong, Sun Peng, Bu Dengpan, Wang Jiaqi

Abstract excerpt

Analysis of the full-length 18S rRNA gene sequences of rumen ciliates is more reliable for taxonomical classification and diversity assessment than the analysis of partial hypervariable regions only. The objective of this study was to develop new oligonucleotide primers targeting the full-length 18S rRNA genes of rumen ciliates, and to evaluate the effect of different sources of dietary fiber (corn stover or a...

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PMID: 26319789
DOI: 10.1007/s00284-015-0898-3

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