Article
Phenotypic and molecular characterization of CTX-M extended-spectrum beta-lactamase-producing Escherichia coli isolates in Shiraz, Iran.
Revista da Sociedade Brasileira de Medicina Tropical - 1 Jan 2000
Zamani Khosrow, Emami Amir, Bazargani Abdollah, Moattari Afagh
Abstract excerpt
INTRODUCTION: The aim of this study was to detect the prevalence of the extended-spectrum beta-lactamase (ESBL)-encoding CTX-M gene in Escherichia coliisolates. METHODS: Phenotypic screening of 376 E. coli isolates for ESBL was conducted using disk diffusion. ESBL-producing isolates were tested using PCR and specific primers. The bla(CTX-M) cluster was identified using the RFLP method, and its genotype was...
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