Article
Active site cleft mutants of Os9BGlu31 transglucosidase modify acceptor substrate specificity and allow production of multiple kaempferol glycosides.
Biochimica et biophysica acta - 1 Jul 2015
Komvongsa Juthamath, Luang Sukanya, Marques Joaquim V, Phasai Kannika, Davin Laurence B, Lewis Norman G, Ketudat Cairns James R
Abstract excerpt
BACKGROUND: Rice Os9BGlu31 is a transglucosidase that can transfer glucose to phenolic acids, flavonoids, and phytohormones. Os9BGlu31 displays a broad specificity with phenolic 1-O-β-D-glucose esters acting as better glucose donors than glucosides, whereas the free phenolic acids of these esters...
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