Article
Cryopreservation of embryogenic tissues from mature holm oak trees.
Cryobiology - 1 Jun 2015
Barra-Jiménez Azahara, Aronen Tuija S, Alegre Jesús, Toribio Mariano
Abstract excerpt
The development of a vitrification method for cryopreservation of embryogenic lines from mature holm oak (Quercus ilex L.) trees is reported. Globular embryogenic clusters of three embryogenic lines grown on gelled medium, and embryogenic clumps of one line collected from liquid cultures, were used as samples. The effect of both high-sucrose preculture and dehydration by incubation in the PVS2 solution for 30-90...
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