Article
Molecular and Biochemical Analysis of Chalcone Synthase from Freesia hybrid in flavonoid biosynthetic pathway.
PloS one - 1 Jan 2015
Sun Wei, Meng Xiangyu, Liang Lingjie, Jiang Wangshu, Huang Yafei, He Jing, Hu Haiyan, Almqvist Jonas, Gao Xiang, Wang Li
Abstract excerpt
Chalcone synthase (CHS) catalyzes the first committed step in the flavonoid biosynthetic pathway. In this study, the cDNA (FhCHS1) encoding CHS from Freesia hybrida was successfully isolated and analyzed. Multiple sequence alignments showed that both the conserved CHS active site residues and CHS signature sequence were found in the deduced amino acid sequence of FhCHS1. Meanwhile, crystallographic analysis...
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