Article
Molecular characterization of viable Legionella spp. in cooling tower water samples by combined use of ethidium monoazide and PCR.
Microbes and environments - 1 Jan 2015
Inoue Hiroaki, Fujimura Reiko, Agata Kunio, Ohta Hiroyuki
Abstract excerpt
Viable Legionella spp. in environmental water samples were characterized phylogenetically by a clone library analysis combining the use of ethidium monoazide and quantitative PCR. To examine the diversity of Legionella spp., six cooling tower water samples and three bath water samples were collected and analyzed. A total of 617 clones were analyzed for their 16S rRNA gene sequences and classified into 99...
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