Article
Characterization of human adipose-derived stem cells cultured in autologous serum after subsequent passaging and long term cryopreservation.
Journal of stem cells - 1 Jan 2014
Bogdanova Ance, Berzins Uldis, Nikulshin Sergey, Skrastina Dace, Ezerta Agnese, Legzdina Diana, Kozlovska Tatjana
Abstract excerpt
The aim of this study was to evaluate human adipose-derived stem cells (ASCs) from passage 2 (P2) to P8 cultured in medium containing 5% autologous serum (AS) after a long-term cryopreservation with regards to their surface marker expression, differentiation potential, and immunosuppressive effect in vitro. 8-color flow cytometry and real time PCR were used to determine mesenchymal stem cell (MSC) surface marker...
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