Article
Sugarcane genome sequencing by methylation filtration provides tools for genomic research in the genus Saccharum.
The Plant journal : for cell and molecular biology - 1 Jul 2014
Grativol Clícia, Regulski Michael, Bertalan Marcelo, McCombie W Richard, da Silva Felipe Rodrigues, Zerlotini Neto Adhemar, Vicentini Renato, Farinelli Laurent, Hemerly Adriana Silva, Martienssen Robert A, Ferreira Paulo Cavalcanti Gomes
Abstract excerpt
Many economically important crops have large and complex genomes that hamper their sequencing by standard methods such as whole genome shotgun (WGS). Large tracts of methylated repeats occur in plant genomes that are interspersed by hypomethylated gene-rich regions. Gene-enrichment strategies based on methylation profiles offer an alternative to sequencing repetitive genomes. Here, we have applied methyl...
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