Article
Tetra-repeat microsatellite markers for the masu salmon (Oncorhynchus masou masou) and its application in cross-subspecies amplification.
International journal of molecular sciences - 21 Nov 2013
Yamamoto Shoichiro, Kurokawa Tadahide, Sekino Masashi, Yasuike Motoshige, Saitoh Kenji
Abstract excerpt
We developed tetranucleotide-repeat microsatellite markers for the masu salmon (Oncorhynchus masou) complex. 454 pyrosequencing was used to discover repeat motifs, and seven polymorphic microsatellite-primer sets were identified. The number of alleles detected at each locus ranged from four to 24 and the expected heterozygosity varied from 0.57 to 0.92. Cross-subspecies amplification for O. m. masou, O. m....
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