Article
Identification and characterization of a novel trehalose synthase gene derived from saline-alkali soil metagenomes.
PloS one - 1 Jan 2013
Jiang Ling, Lin Ming, Zhang Yang, Li Yanping, Xu Xian, Li Shuang, He Huang
Abstract excerpt
A novel trehalose synthase (TreS) gene was identified from a metagenomic library of saline-alkali soil by a simple activity-based screening system. Sequence analysis revealed that TreS encodes a protein of 552 amino acids, with a deduced molecular weight of 63.3 kDa. After being overexpressed in Escherichia coli and purified, the enzymatic properties of TreS were investigated. The recombinant TreS displayed its...
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