Article
Consequences of growth media, gene copy number, and regulatory mutations on the expression of the PRB1 gene of Saccharomyces cerevisiae.
Genetics - 1 Jan 1990
Moehle C M, Jones E W
Abstract excerpt
Glucose represses PRB1 expression at the level of transcription. However, release from glucose repression initially does not result in accumulation of protease B (PrB) activity despite transcriptional derepression. PrB activity accumulates only upon a second transcriptional derepression as the cells approach stationary phase. Increasing the PRB1 gene dosage on 2 mu-based plasmids does not overcome glucose...
Topics
- Culture Media
- Dosage Compensation, Genetic
- Gene Expression Regulation, Enzymologic
- Gene Expression Regulation, Fungal
- Genes, Fungal
- Glucose
- Kinetics
- Mutation
- Phenotype
- Saccharomyces cerevisiae
- Serine Endopeptidases