Article
The identification of two Trypanosoma cruzi I genotypes from domestic and sylvatic transmission cycles in Colombia based on a single polymerase chain reaction amplification of the spliced-leader intergenic region.
Memorias do Instituto Oswaldo Cruz - 1 Nov 2013
Villa Lina Marcela, Guhl Felipe, Zabala Daniel, Ramírez Juan David, Urrea Daniel Alfonso, Hernández Diana Carolina, Cucunubá Zulma, Montilla Marleny, Carranza Julio César, Rueda Karina, Trujillo Jorge Eduardo, Vallejo Gustavo Adolfo
Abstract excerpt
A single polymerase chain reaction (PCR) reaction targeting the spliced-leader intergenic region of Trypanosoma cruzi I was standardised by amplifying a 231 bp fragment in domestic (TcIDOM) strains or clones and 450 and 550 bp fragments in sylvatic strains or clones. This reaction was validated using 44 blind coded samples and 184 non-coded T. cruzi I clones isolated from sylvatic triatomines and the...
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