Article
[The identification of esterase D isoenzyme (EsD) phenotypes in the dental pulp by isoelectric focusing].
Minerva stomatologica - 1 Jun 1990
De Leo D, Tagliaro F
Abstract excerpt
An identification method based on the isoelectrofocusing (IEF) assay of the EsD phenotypes in dental cavity pulp is reported. The IEF has been carried out on polyacrylamide gel (T = 5% and C = 3%), using ampholines ranging pH 4.5-5.4. A pre-focusing of 20 min at 2,000 V, 100 mA and 8 W was used; focusing conditions were: 2,000 V, unlimited current and W, for 75 min at 4 degrees C. Electropherograms were read...
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