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Article

Phenotyping of erythrocyte acid phosphatase and esterase D by high field strength isoelectric focusing on cellulose acetate membrane.

Electrophoresis - 1 Apr 1990

Kane M, Yamamoto Y, Yamada M, Fukunaga T, Tatsuno Y

Abstract excerpt

Phenotyping of erythrocyte acid phosphatase (EAP) and esterase D (ESD) by cellulose acetate membrane isoelectric focusing (CAM-IEF) under a nonequilibrium condition is described. In an attempt to improve the method of CAM-IEF, we shortened the electrode distance to provide a higher field strength at a given (low) voltage. Various carrier ampholytes for EAP typing and various chemical separators for ESD typing...

Topics

Acid Phosphatase
Carboxylesterase
Carboxylic Ester Hydrolases
Cellulose
Erythrocytes
Humans
Hydrogen-Ion Concentration
Isoelectric Focusing
Phenotype

Source

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Identifiers

PMID: 2340827
DOI: 10.1002/elps.1150110409

Genes

ESD

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2340827-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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Phenotyping of erythrocyte acid phosphatase and esterase D by high field strength isoelectric focusing on cellulose acetate membrane.PMID 2340827Read ↗

Electrophoretic phenotyping of erythrocyte enzymes.Semantic proximity Read ↗

Studies on the use of isoelectric focusing as a method of phenotyping erythrocyte acid phosphatase.Semantic proximity Read ↗

The simultaneous phenotyping of erythrocyte acid phosphatase, esterase D, phosphoglucomutase and adenosine deaminase by isoelectric focusing.Semantic proximity Read ↗

Evaluation of a nonequilibrium isoelectric focusing (IEF) method for the simultaneous typing of esterase D (EsD), red cell acid phosphatase (AcP1), phosphoglucomutase (PGM1), adenylate kinase (AK), and adenosine deaminase (ADA).Semantic proximity Read ↗

Esterase D (ESD EC 3.1.1.1) polymorphism in hemolysates and bloodstains. II. Comparison of ESD stability in dried bloodstains in relation to the temperature of storage and the type and character of substrate.Semantic proximity Read ↗

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Esterase D (ESD, EC 3.1.1.1) polymorphism in hemolysates and bloodstains. I. Usefulness of selected methods of ESD phenotyping.Semantic proximity Read ↗

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