Article
Identification of a virB10 protein aggregate in the inner membrane of Agrobacterium tumefaciens.
Journal of bacteriology - 1 Sept 1990
Ward J E, Dale E M, Nester E W, Binns A N
Abstract excerpt
Products of the virB operon are proposed components of a membrane-associated T-DNA transport apparatus in Agrobacterium tumefaciens. Here we identified the virB10 gene product and raised specific antiserum to the protein. While the virB10 reading frame contains two potential ATG translation start sites located 32 codons apart, we found that only the downstream ATG was required for efficient VirB10 synthesis....
Topics
- Amino Acid Sequence
- Bacterial Proteins
- Base Sequence
- Cell Membrane
- Cloning, Molecular
- Codon
- Genetic Vectors
- Immunoblotting
- Molecular Sequence Data
- Mutation
- Oligonucleotide Probes