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Article

Isolation and characterization of Rhodobacter capsulatus mutants defective in oxygen regulation of the puf operon.

Journal of bacteriology - 1 Aug 1990

Narro M L, Adams C W, Cohen S N

Abstract excerpt

cis-acting mutations that affect regulation of the Rhodobacter capsulatus puf operon by oxygen were isolated by placing the mutagenized puf regulatory region 5' to a promoterless Tn5 neo gene, which encodes resistance to kanamycin (Kmr). R. capsulatus mutants that failed to show wild-type repression of KMr by oxygen were selected and analyzed. Four independent clones contained point mutations, three of which were...

Topics

Base Sequence
Cloning, Molecular
Gene Expression Regulation, Bacterial
Molecular Sequence Data
Mutation
Oligonucleotide Probes
Operon
Oxygen
Plasmids
Promoter Regions, Genetic
Restriction Mapping

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Rhodopseudomonas

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Identifiers

PMID: 2376568
DOI: 10.1128/jb.172.8.4549-4554.1990
PMCID: PMC213287

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2376568-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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