Article
A duplex PCR for rapid and simultaneous detection of Brucella spp. in human blood samples.
24 May 2013
Abstract excerpt
OBJECTIVE: To design a duplex PCR for rapid and simultaneous detection of Brucella species. in human blood samples. METHODS: Fifty-two peripheral bloods samples were collected from suspicious patients with brucellosis. Following DNA extraction, PCR assay were performed, using three primers that could simultaneously identify and differentiate three major species of pathogenic Brucella in humans and animals....
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