Article
Phenotypic correction of factor IX deficiency in skin fibroblasts of hemophilic dogs.
Proceedings of the National Academy of Sciences of the United States of America - 1 Jul 1990
Axelrod J H, Read M S, Brinkhous K M, Verma I M
Abstract excerpt
Primary skin fibroblasts from hemophilic dogs were transduced by recombinant retrovirus (LNCdF9L) containing a canine factor IX cDNA. High levels of biologically active canine factor IX (1.0 micrograms per 10(6) cells per 24 hr) were secreted in the medium. The level of factor IX produced increased substantially if the cells were stimulated by basic fibroblast growth factor during infection. Additionally, we also...
Topics
- Amino Acid Sequence
- Animals
- Base Sequence
- Cells, Cultured
- Cloning, Molecular
- DNA
- Dog Diseases
- Dogs
- Factor IX
- Fibroblasts
- Gene Library
- Genetic Therapy