Article
Porcine E. coli: virulence-associated genes, resistance genes and adhesion and probiotic activity tested by a new screening method.
PloS one - 1 Jan 2013
Schierack Peter, Rödiger Stefan, Kuhl Christoph, Hiemann Rico, Roggenbuck Dirk, Li Ganwu, Weinreich Jörg, Berger Enrico, Nolan Lisa K, Nicholson Bryon, Römer Antje, Frömmel Ulrike, Wieler Lothar H, Schröder Christian
Abstract excerpt
We established an automated screening method to characterize adhesion of Escherichia coli to intestinal porcine epithelial cells (IPEC-J2) and their probiotic activity against infection by enteropathogenic E. coli (EPEC). 104 intestinal E. coli isolates from domestic pigs were tested by PCR for the occurrence of virulence-associated genes, genes coding for resistances to antimicrobial agents and metals, and for...
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