Article
Usefulness of two methods of isoelectric focusing for the erythrocyte acid phosphatase phenotypes determination in human bloodstains.
Forensic science international - 1 Jan 2000
Pawlowski R, Raszeja S, Komocka G, Kuczyński S
Abstract excerpt
The authors tried to compare the usefulness of the isoelectric focusing of EAP in bloodstains on 0.2 mm polyacrylamide gel with their method of determination of the enzyme on 1 mm polyacrylamide gel. Both methods turned out to be useful but better results were obtained on 0.2 mm gel. Isoelectric focusing on the ultra-thin gel is more sensitive; it gives clear enzyme strips, takes less time (30 min) and demands...
Topics
- Acid Phosphatase
- Blood Stains
- Electrophoresis, Polyacrylamide Gel
- Erythrocytes
- Humans
- Hydrogen-Ion Concentration
- Isoelectric Focusing
- Isoenzymes
- Phenotype
- Reference Values