Article
Phenotypic changes associated with RNA interference silencing of chalcone synthase in apple (Malus × domestica).
The Plant journal : for cell and molecular biology - 1 May 2013
Dare Andrew P, Tomes Sumathi, Jones Midori, McGhie Tony K, Stevenson David E, Johnson Ross A, Greenwood David R, Hellens Roger P
Abstract excerpt
We have identified in apple (Malus × domestica) three chalcone synthase (CHS) genes. In order to understand the functional redundancy of this gene family RNA interference knockout lines were generated where all three of these genes were down-regulated. These lines had no detectable anthocyanins and radically reduced concentrations of dihydrochalcones and flavonoids. Surprisingly, down-regulation of CHS also led...
Topics
- Acyltransferases
- Biological Transport
- Cell Shape
- Chalcones
- Enzyme Activation
- Flavanones
- Fruit
- Gene Expression Regulation, Enzymologic
- Gene Expression Regulation, Plant
- Genes, Plant
- Genetic Complementation Test
- Indoleacetic Acids