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High-throughput isolation of giant viruses of the Mimiviridae and Marseilleviridae families in the Tunisian environment.

Environmental microbiology - 1 Jul 2013

Boughalmi Mondher, Saadi Hanene, Pagnier Isabelle, Colson Philippe, Fournous Ghislain, Raoult Didier, La Scola Bernard

Abstract excerpt

Giant viruses of the Megavirales order have been recently isolated from aquatic environments and have long been neglected because they are removed from samples during viral purification for viral metagenomic studies. Due to bacterial overgrowth and susceptibility to high concentrations of antibiotics, isolation by amoeba co-culture has a low efficiency and is highly time-consuming. Thus, few environments have...

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PMID: 23298151
DOI: 10.1111/1462-2920.12068

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