Article
Phylogenetic conservation of arylsulfatases. cDNA cloning and expression of human arylsulfatase B.
The Journal of biological chemistry - 25 Feb 1990
Peters C, Schmidt B, Rommerskirch W, Rupp K, Zühlsdorf M, Vingron M, Meyer H E, Pohlmann R, von Figura K
Abstract excerpt
A 2.2-kilobase cDNA clone for human arylsulfatase B (ASB) and several genomic clones were isolated and sequenced. The deduced amino acid sequence of 533 amino acids contains a 41-amino acid N-terminal signal peptide and a mature polypeptide of 492 amino acid residues. Overexpression of ASB in transfected baby hamster kidney (BHK) cells resulted in up to 68-fold higher ASB activity than in untransfected BHK cells....
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