Article
Validation of a cheap and simple nondestructive method for obtaining AFLPs and DNA sequences (mitochondrial and nuclear) in amphibians.
Molecular ecology resources - 1 Nov 2012
Gallardo C E, Correa C, Morales P, Sáez P A, Pastenes L, Méndez M A
Abstract excerpt
The use of nondestructive methods for obtaining DNA from amphibians (e.g. buccal swabs) allows genetic studies to be performed without affecting the survival of the studied individuals. In this study, we compared two methods of nondestructive DNA sampling, buccal swabs and interdigital membrane or toe-clipping, in several amphibian species of different size: Rhinella spinulosa, R. atacamensis, six species of the...
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