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Article

Identification of domains in p27 auxiliary replicase protein essential for its association with the endoplasmic reticulum membranes in Red clover necrotic mosaic virus.

Virology - 10 Nov 2012

Kusumanegara Kusumawaty, Mine Akira, Hyodo Kiwamu, Kaido Masanori, Mise Kazuyuki, Okuno Tetsuro

Abstract excerpt

Positive-strand RNA viruses require host intracellular membranes for replicating their genomic RNAs. In this study, we determined the domains and critical amino acids in p27 of Red clover necrotic mosaic virus (RCNMV) required for its association with and targeting of ER membranes in Nicotiana benthamiana plants using a C-terminally GFP-fused and biologically functional p27. Confocal microscopy and...

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PMID: 22898643
DOI: 10.1016/j.virol.2012.07.017

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