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Article

Inactivation of Met471Cys tyramine β-monooxygenase results from site-specific cysteic acid formation.

Biochemistry - 25 Sept 2012

Osborne Robert L, Zhu Hui, Iavarone Anthony T, Hess Corinna R, Klinman Judith P

Abstract excerpt

Tyramine β-monooxygenase (TβM), the insect homologue of dopamine β-monooxygenase, is a neuroregulatory enzyme that catalyzes the β-hydroxylation of tyramine to yield octopamine. Mutation of the methionine (Met) ligand to Cu(M) of TβM, Met471Cys, yielded a form of TβM that is catalytically active but susceptible to inactivation during turnover [Hess, C. R., Wu, Z., Ng, A., Gray, E. E., McGuirl, M. M., and Klinman,...

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PMID: 22891760
DOI: 10.1021/bi300456f
PMCID: PMC3567250

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