Article
Porcine reproductive and respiratory syndrome virus: interlaboratory ring trial to evaluate real-time reverse transcription polymerase chain reaction detection methods.
Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc - 1 Sept 2012
Wernike Kerstin, Bonilauri Paolo, Dauber Malte, Errington Jane, LeBlanc Neil, Revilla-Fernández Sandra, Hjulsager Charlotte, Isaksson Mats, Stadejek Tomasz, Beer Martin, Hoffmann Bernd
Abstract excerpt
To compare the real-time reverse transcription quantitative polymerase chain reaction (RT-qPCR) assays used for the diagnosis of Porcine reproductive and respiratory syndrome virus (PRRSV), a Europe-wide interlaboratory ring trial was conducted. A variety of PRRSV strains including North American (NA) and European (EU) genotype isolates were analyzed by the participants. Great differences regarding qualitative...
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