Article
Critical analysis of rhinovirus RNA load quantification by real-time reverse transcription-PCR.
Journal of clinical microbiology - 1 Sept 2012
Schibler Manuel, Yerly Sabine, Vieille Gaël, Docquier Mylène, Turin Lara, Kaiser Laurent, Tapparel Caroline
Abstract excerpt
Rhinoviruses are the most frequent cause of human respiratory infections, and quantitative rhinovirus diagnostic tools are needed for clinical investigations. Although results obtained by real-time reverse-transcription PCR (RT-PCR) assays are frequently converted to viral RNA loads, this presents several limitations regarding accurate virus RNA quantification, particularly given the need to reliably quantify all...
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