Article
Use of fluorescent protein to analyse recombination at three loci in Neurospora crassa.
Fungal genetics and biology : FG & B - 1 Aug 2012
Bowring Frederick J, Yeadon P Jane, Catcheside David E A
Abstract excerpt
We have inserted a histone H1-GFP fusion gene adjacent to three loci on different chromosomes of Neurospora crassa and made mating pairs in which a wild type version of GFP is crossed to one with a mutation in the 5' end of GFP. The loci are his-3, am and his-5, chosen because recombination mechanisms appear to differ between his-3 and am, and because crossing over adjacent to his-5, like his-3, is regulated by...
Topics
- Alleles
- Chromosomes, Fungal
- Crossing Over, Genetic
- Fungal Proteins
- Gene Conversion
- Genetic Loci
- Green Fluorescent Proteins
- Hemizygote
- Histones
- Mutation
- Neurospora crassa
