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Article

Flow cytometric analysis of somaclonal variation in lineages of Hosta sports detects polyploidy and aneuploidy chimeras.

Plant biology (Stuttgart, Germany) - 1 Nov 2012

Zonneveld B J M, Pollock W I

Abstract excerpt

Somaclonal variation of some 124 specially selected cultivars of Hosta Tratt. (Hostaceae) was investigated. Nuclear DNA contents (2C-value) were measured by flow cytometry of leaves and roots of L1, L2 and L3 layers derived from apical meristems. These values were then converted to inferred ploidies by comparing the measured 2C-values and ploidy with those of the parent plant. During tissue-culture propagation,...

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PMID: 22676855
DOI: 10.1111/j.1438-8677.2012.00584.x

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