Article
Evaluation of distinct freezing methods and cryoprotectants for human amniotic fluid stem cells cryopreservation.
Journal of biomedicine & biotechnology - 1 Jan 2012
Janz Felipe de Lara, Debes Adriana de Aguiar, Cavaglieri Rita de Cássia, Duarte Sérgio Aloísio, Romão Carolina Martinez, Morón Antonio Fernandes, Zugaib Marcelo, Bydlowski Sérgio Paulo
Abstract excerpt
Amniotic fluid (AF) was described as a potential source of mesenchymal stem cells (MSCs) for biomedicine purposes. Therefore, evaluation of alternative cryoprotectants and freezing protocols capable to maintain the viability and stemness of these cells after cooling is still needed. AF stem cells (AFSCs) were tested for different freezing methods and cryoprotectants. Cell viability, gene expression, surface...
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