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Article

DHR123: an alternative probe for assessment of ROS in human spermatozoa

30 Apr 2012

Abstract excerpt

The aim of this study was to assess the potential of dihydrorhodamine 123 (DHR123) to measure oxidative stress produced by human spermatozoa. The results were compared with 2', 7'-dichlorodihydrofluorescein diacetate (DCFH-DA) that is routinely used for assessment of H(2)O(2) produced by spermatozoa. Fluorescence intensity and percentage R123 and DCF positive sperm were measured by flow cytometry. The optimal...

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PMID: 22545706
DOI: 10.3109/19396368.2012.681420

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