Article
Discovery of pod shatter-resistant associated SNPs by deep sequencing of a representative library followed by bulk segregant analysis in rapeseed.
PloS one - 1 Jan 2012
Hu Zhiyong, Hua Wei, Huang Shunmou, Yang Hongli, Zhan Gaomiao, Wang Xinfa, Liu Guihua, Wang Hanzhong
Abstract excerpt
BACKGROUND: Single nucleotide polymorphisms (SNPs) are an important class of genetic marker for target gene mapping. As of yet, there is no rapid and effective method to identify SNPs linked with agronomic traits in rapeseed and other crop species. METHODOLOGY/PRINCIPAL FINDINGS: We demonstrate a novel method for identifying SNP markers in rapeseed by deep sequencing a representative library and performing bulk...
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