Article
Comprehensive DNA copy number profile and BAC library construction of an Indian individual.
Gene - 1 Jun 2012
Chakrabarty Sanjiban, D'Souza Reena R, Bellampalli Ravishankara, Rotti Harish, Saadi Abdul V, Gopinath Puthiya M, Acharya Raviraja V, Govindaraj Periyasamy, Thangaraj Kumarasamy, Satyamoorthy Kapaettu
Abstract excerpt
Bacterial artificial chromosomes (BACs) are used in genomic variation studies due to their capacity to carry a large insert, their high clonal stability, low rate of chimerism and ease of manipulation. In the present study, an attempt was made to create the first genomic BAC library of an anonymous Indian male (IMBL4) consisting of 100,224 clones covering the human genome more than three times. Restriction...
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