Article
Exploitation of a turbot (Scophthalmus maximus L.) immune-related expressed sequence tag (EST) database for microsatellite screening and validation.
Molecular ecology resources - 1 Jul 2012
Navajas-Pérez R, Robles F, Molina-Luzón M J, De La Herrán R, Alvarez-Dios J A, Pardo B G, Vera M, Bouza C, Martínez P
Abstract excerpt
In this study, we identified and characterized 160 microsatellite loci from an expressed sequence tag (EST) database generated from immune-related organs of turbot (Scophthalmus maximus). A final set of 83 new polymorphic microsatellites were validated after the analysis of 40 individuals of Atlantic origin including both wild and farmed individuals. The allele number and the expected heterozygosity ranged from 2...
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