Article
RNA binding is more critical to the suppression of silencing function of Cucumber mosaic virus 2b protein than nuclear localization.
RNA (New York, N.Y.) - 1 Apr 2012
González Inmaculada, Rakitina Daria, Semashko Maria, Taliansky Michael, Praveen Shelly, Palukaitis Peter, Carr John P, Kalinina Natalia, Canto Tomás
Abstract excerpt
Previously, we found that silencing suppression by the 2b protein and six mutants correlated both with their ability to bind to double-stranded (ds) small RNAs (sRNAs) in vitro and with their nuclear/nucleolar localization. To further discern the contribution to suppression activity of sRNA binding and of nuclear localization, we have characterized the kinetics of in vitro binding to a ds sRNA, a single-stranded...
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