Article
Pyrosequencing of a short fragment of the amelogenin gene for gender identification.
Molecular biology reports - 1 Jun 2012
Li Shujin, Feng Ting, Fu Lihong, Li Zhenhua, Lou Chunguang, Zhang Xiaojing, Ma Chunling, Cong Bin
Abstract excerpt
We report a pyrosequencing method for detecting a short amelogenin fragment to aid the gender identification. The PCR products (44/45 bp), including primers and target sequence (4/5 bp) consisting of three point mutations and one indel mutation, were sequenced by the pyrosequencing method. 100 randomly chosen DNA samples of healthy donors were analyzed with this method, and all of them were correctly typed. The...
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