Article
"YFlag"--a single-base extension primer based method for gender determination.
Journal of forensic sciences - 1 Jan 2015
Allwood Julia S, Harbison Sally Ann
Abstract excerpt
Assigning the gender of a DNA contributor in forensic analysis is typically achieved using the amelogenin test. Occasionally, this test produces false-positive results due to deletions occurring on the Y chromosome. Here, a four-marker "YFlag" method is presented to infer gender using single-base extension primers to flag the presence (or absence) of Y-chromosome DNA within a sample to supplement forensic STR...
Topics
- Cell Cycle Proteins
- Chromosomes, Human, Y
- DNA Fingerprinting
- DNA Primers
- Female
- Genes, sry
- Genetic Markers
- Humans
- Male
- Multiplex Polymerase Chain Reaction
- Polymorphism, Single Nucleotide