Article
Mitogen-activated protein kinase 5, a novel molecular marker for the identification and detection of Trypanozoon species.
Acta tropica - 1 May 2012
Wang Ming-Hui, Wen Yan-Zi, Wei Ying, Lai De-Hua, Bengaly Zakaria, Desquesnes Marc, Lun Zhao-Rong
Abstract excerpt
Based on the sequence of mitogen-activated protein kinase 5 (TbMAPK5) gene, we have developed a specific PCR method which can delineate the species within the Trypanozoon subgenus from other parasites or host DNA conveniently. In view of further application in field studies, we performed loop-mediated isothermal amplification (LAMP) employing filter paper flecked with rodent blood. Our data showed that...
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