Article
A high-throughput apple SNP genotyping platform using the GoldenGate™ assay.
Gene - 25 Feb 2012
Khan M Awais, Han Yuepeng, Zhao Youfu Frank, Korban Schuyler S
Abstract excerpt
EST data generated from 14 apple genotypes were downloaded from NCBI and mapped against a reference EST assembly to identify Single Nucleotide Polymorphisms (SNPs). Mapping of these SNPs was undertaken using 90% of sequence similarity and minimum coverage of four reads at each SNP position. In total, 37,807 SNPs were identified with an average of one SNP every 187 bp from a total of 6888 unique EST contigs....
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