Article
The use of whole-cell protein profile analysis by SDS-PAGE as an accurate tool to identify species and subspecies of coagulase-negative staphylococci.
APMIS : acta pathologica, microbiologica, et immunologica Scandinavica - 1 Jan 2012
Santos Odelta Dos, De Resende Mariah Costa Carvalho, De Mello Ariéle Lima, Frazzon Ana Paula Guedes, D'Azevedo Pedro Alves
Abstract excerpt
We used sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) as a tool to characterize coagulase-negative staphylococci (CoNS). Of 253 clinical isolates and 10 control strains, five species and four subspecies were analyzed. All the isolates were identified using conventional phenotypic tests and SDS-PAGE. Discrepant results between these methods, as well as less common species and subspecies,...
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