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Article

Carbohydrate binding activities of Bradyrhizobium japonicum. II. Isolation and characterization of a galactose-specific lectin.

The Journal of cell biology - 1 Oct 1990

Ho S C, Schindler M, Wang J L

Abstract excerpt

Extracts of Bradyrhizobium japonicum were fractionated on Sepharose columns covalently derivatized with lactose. Elution of the material that was specifically bound to the affinity column with lactose yielded a protein of Mr approximately 38,000. Isoelectric focusing of this sample yielded two spots with pI values of 6.4 and 6.8. This protein specifically bound to galactose-containing glycoconjugates, but did not...

Topics

Binding, Competitive
Carbohydrate Metabolism
Carbohydrate Sequence
Chelating Agents
Chromatography, Affinity
Galactose
Hydrogen-Ion Concentration
Isoelectric Point
Lactose
Lectins
Molecular Sequence Data

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Molecular Weight

Mutation

Rhizobiaceae

Sepharose

Source

Open in PubMed Open full text

Identifiers

PMID: 2211830
DOI: 10.1083/jcb.111.4.1639
PMCID: PMC2116231

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2211830-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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