Article
Interspecific somatic hybrids between Cyclamen persicum and C. coum, two sexually incompatible species.
Plant cell reports - 1 Apr 2012
Prange Anika Nadja Sabine, Bartsch Melanie, Meiners Julia, Serek Margrethe, Winkelmann Traud
Abstract excerpt
By applying polyethylene glycol (PEG)-mediated protoplast fusion, the first somatic hybrids were obtained between Cyclamen persicum (2n = 2x = 48) and C. coum (2n = 2x = 30)-two species that cannot be combined by cross breeding. Heterofusion was detected by double fluorescent staining with fluorescein diacetate and scopoletin. The highest heterofusion frequencies (of about 5%) resulted from a protocol using a...
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